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This example of a multiple scatter plot includes: Common log scale Y axis with major and minor tick marks. Custom sized symbols using an incrementing earthtone color scheme. This example of a box plot includes: Variable box widths expressing another variable dimension. Tick mark direction pointing out. X axis tick labels using a category axis scale. A Tukey box plot with mean value lines.

Custom fill colors. This line and scatter plot with error bars includes: A scatter plot of column averaged data points, with Y error bars computed from the standard deviations of the data.

A top X axis with tick marks turned off. Y axis with a linear axis scale. A left Y axis title. A left Y axis with major tick marks. Numeric major tick labels. An X axis with a linear axis scale. A bottom X axis title. A right Y axis with tick marks turned off. An automatically generated legend. A reference line. Pie Chart Example Use Pie charts to display a quick comparison of ratios in a data set.

The example figure displays: Slice fills Text labels Exploded slices. Polar Plot Example Use Polar plots to display modular data such as average monthly temperatures, or satellite positioning in the sky over a period of time. The following example includes: Major and minor contour lines Contour labels.

Because hidden lines are eliminated, waterfall plots are useful for showing trends of line plots. The following example includes: Incremented line fill color Eliminated “hidden” lines. Area Plot Example Area plots are 2D line plots with regions below or between curves filled with a color or pattern.

Most commonly, an area plot is a line plot with shading that descends to the axis. You can add shade below a curve and shade in different directions. You can also identify intersecting sections. This example consists of two plots, and includes: A simple bar chart using hairline bars. A multiple area plot using the X many Y data format. Customer Service If you have any questions concerning your shipment or account, contact your local office.

For more information, see Contacting Systat Software, Inc. Please have your serial number ready for identification when calling. Training Seminars Systat Software, Inc. All seminars feature hands-on workshops. Systat seminars will be offered in major U. Tell Us Your Thoughts Your comments are important.

Please send us a letter and let us know about new and interesting applications using Systat products. Write to Systat Software, Inc. Customers may call Technical Support for assistance in using Systat products or for installation help for one of the supported hardware environments. In the U. Pacific Time Fax: E-mail: techsupport systat. Contacting Systat Software, Inc. If you would like to be on our mailing list, contact one of our offices or distributors below.

We will send you a copy of our newsletter and let you know about Systat Software, Inc. Systat Software, Inc. References We have found the following references very useful for graph design and layout.

Brent Charland, Ph. SigmaPlot for Scientists. Brown Communications, Inc. Cleveland, William S. The Elements of Graphing Data. Monterey, Calif. Kosslyn, Stephen M. Elements of Graph Design. New York: W. Freeman and Company. Tufte, Edward R. The Visual Display of Quantitative Information. Cheshire, Conn. Available from Science News Books, N. NW, Washington, D. Illustrating Science: Standards for Publication. Notebook files contain all of your data and graphs, and are organized within the Notebook Manager.

This covers: Notebook Manager organization. Saving your work. Creating notebooks and adding notebook items. For more information, see Working with Sections in the Notebook Manager. Opening notebooks and notebook items. Copying, pasting, and deleting notebook items. Notebook Manager Overview The Notebook Manager is a dockable or floating window that displays all open notebooks. The first time you see the Notebook Manager, it appears with one open notebook, which contains one section.

That section contains one empty worksheet. Contents of th Notebook Manager appear as a tree structure, similar to Windows Explorer. Each open notebook appears as the top level, with one or more sections at the second level, and one or more items at the third level. Within each section, you can create one worksheet and an unlimited number of graph pages, reports, equations and macros.

The most recently opened notebook file appears at the top of the Notebook Manager. Modified Notebook Names. An asterisk next to an item in the Notebook Manager indicates that the item has been modified since the last time you saved the notebook. Notebook Item Names. The default startup notebook is named Notebook1.

It contains one notebook section, Section 1, and one worksheet, Data 1. When you save your notebook file, the name of the file appears at the top of the Notebook Manager window. Notebook files use a. The default names given to notebook sections and items are, Section number , Data number or Excel number and Report number. Regression equations are named when they are created. New items are numbered sequentially. All opened notebooks appear in the Notebook Manager. You can navigate through the different open notebooks by selecting them in the Notebook Manager.

You can hide them by clicking the Close button on the upper right-hand corner of the Notebook window; however, this does not close the item. It only hides it from view. To close notebook, use the File menu. To open a notebook:. From the File menu, click Open.

The Open dialog box appears. Select a notebook. The notebook appears in the Notebook Manager. To close a notebook:. Select the notebook to close in the Notebook Manager. Right-click, and from the shortcut menu, click Close Notebook. You can also choose Close Notebook from the File menu. Docked with summary information hidden. Floating with summary information in view. Floating with summary information hidden. Docked and collapsed.

To undock the Notebook Manager, double-click the title bar and drag it to the desired location. To dock the Notebook Manager and move it back to its original position, double-click the title bar again.

To view summary information, click Show summary information. To hide it, click Hide summary information. To collapse the Notebook Manager, click the arrow button on the top right-hand corner of the Notebook Manager when docked.

To view again, click the graph icon. To drag and drop the Notebook manager, click the title bar and drag the Notebook Manager anywhere on the SigmaPlot desktop. Saving Your Work Be sure to save your work at regular intervals. To save a notebook file for the first time:. Click the Save button. The Save As dialog box appears. Navigate to the directory where you want to save your notebook.

Type a name for the notebook in the File Name text box. Click Save to save the notebook file and close the Save As dialog box. To save changes with the same name and path:. Click the Save button on the Standard toolbar. Your file is saved. To save to a new name and path:. On the File menu, click Save As. Printing Selected Notebook Items You can print active worksheets, graph pages, reports, and selected notebook items by clicking the Print button on the Standard toolbar.

You can print individual or multiple items from the notebook, including entire sections. Select one or more items or sections from the notebook.

Click the Print button on the Standard toolbar to print the worksheet using all the default settings. To set printing options before printing a report, graph page, or worksheet:. Protecting Notebooks To ensure security of notebook contents, you can lock notebooks using a password. This is particularly useful if two or more users are using the same version of SigmaPlot.

You can also use a password to send confidential data to other SigmaPlot users. Setting a Password To set a password: 1. Select the notebook in the Notebook Manager. On the Tools menu, click Password. The Set Password dialog box appears.

In the New Password box, type a password. In the Reconfirm box, type the password again. Changing or Removing a Password To change or remove a password: 1.

In the Old Password box, type the old password. In the New Password box, type a new password. If you want to remove this password, leave this box and the Reconfirm box, empty. Working with Sections in the Notebook Manager Notebook sections are placeholders in the notebook. They contain notebook items, but no data. However, you can name, open, and close notebook sections.

You can create as many new sections as you want in a notebook. You may also create reports within each section to document the items in each section. Right-click anywhere in the Notebook Manager that you want the new section or item to appear. On the shortcut menu click New, and then select the item to create. The new section or item appears in the Notebook Manager. Copying and Pasting to Create New Sections Another method to create a new notebook section is to copy and paste a section in the notebook window.

Whenever you copy and paste a section, its contents appear at the bottom of the notebook window. SigmaPlot names and numbers the section automatically. For example, if you copy notebook Section 3, the new section is named Copy of Section 3. Copied sections create copies of all items within that section as well. Renaming Notebook Files and Items You can change summary information for all notebook files and items. To change summary information:. If the summary information is hidden on the Notebook Manager, click View summary information.

Select the notebook item and edit as appropriate. In-place Editing Section and Item Names You can change the name of a notebook section or item in the notebook itself without opening the Summary Information dialog box.

To in-place edit:. In the Notebook Manager, click the section or item you want to rename. Click it a second time. Type the new name. Press Enter. The new section or item name appears. Note: To change the name of the notebook, use the Save As dialog box. For more information, see Saving Your Work above. Copying a Page to a Section with No Worksheet If you copy a graph page into an empty section or a section that has no worksheet, you create an independent page.

The independent page retains all its plotted data without the worksheet. You can store the pages from several different sections that have different data together this way.

However, if you ever create or paste a worksheet into a section, all independent pages will revert to plotting the data from the new worksheet. Use independent pages as templates, or to draw or store objects.

You cannot create graphs for an independent page until it is associated with a worksheet and no longer independent. To open a notebook file that is stored on a disk: 1. Click the Open button on the Standard toolbar.

Figure Open Dialog Box. Choose the appropriate drive and directory of the notebook file to open. Double-click the desired notebook file. If you want to open another type of file, choose the type of file from the Files of type list.

Click Open. The opened notebook appears in the Notebook Manager. Opening Worksheets, Reports, and Pages You can open a worksheet, report, or page by double-clicking its icon in the Notebook Manager. You can also right-click the item, and on the shortcut menu, click Open. Open worksheets, pages and report appear in their own window, and in the notebook as a colored icons. Double-clicking an item that is already open brings the items window to the front.

Opening Multiple Items. You can open as many items as your systems memory allows. You can open multiple items from multiple notebooks. The selected item appears highlighted in the Notebook Manager. Copying and Pasting Items in the Notebook Manager You can copy and paste items from one open notebook file to another in the Notebook Manager; however, you cannot copy a worksheet into a notebook section that already contains a worksheet.

Copying and pasting pages and worksheets between sections results in using graph pages as templates. To copy and paste a notebook item:. Right-click the item in the Notebook Manager that you want to copy, and on the shortcut menu, click Copy. Right-click the section where you want to paste the item, and on the shortcut menu, click Paste.

The selected item is pasted to the current notebook and section. Select the item and press Delete. The item is deleted. Items removed from a notebook file using the Delete button are removed permanently. Worksheets are the containers for the data you analyze and graph.

They are spreadsheet-like in appearance but are limited in function, and are column rather than cell oriented. The following figure provides some worksheet definitions: Figure Example of a SigmaPlot Worksheet.

To enter data, you can type in, paste, or import data from other sources. You can also automatically generate and place data in worksheet columns by data transforms and statistical procedures. This chapter covers: Setting worksheet display options. For more information, see Setting Worksheet Display Options below.

Moving around the worksheet. For more information, see Moving Around the Worksheet below. Entering data. For more information, see Entering Data into a Worksheet below. Importing files from other applications. For more information, see Importing Files from Other Applications below. Exporting worksheets. For more information, see Exporting Worksheet Data below.

Viewing worksheet statistics. For more information, see Descriptive Statistics for Worksheets below. Displaying worksheet data. For more information, see Displaying Worksheet Data below.

Formatting worksheets. For more information, see Formatting Worksheets below. Cutting, copying, pasting, moving, and deleting data. Entering and promoting column and row titles. Removing outliers and other data. For more information, see Removing Outliers and Other Data below. Using Excel Workbooks in SigmaPlot. Printing worksheets. For more information, see Printing Worksheets below. Opening a New and Multiple Worksheets You can open as many worksheets as desired. Each worksheet you open is assigned to its own notebook section.

To open a worksheet:. The New dialog box appears. Figure Selecting Worksheet from the New dialog box. Select Worksheet from the New drop-down list. Select SigmaPlot from the Type list. A new worksheet appears. Saving Worksheets to Notebooks Worksheets are saved to notebook files. To save data for the current worksheet to a notebook file: 1. From the menus select: File Save. If you are saving the notebook for the first time, the Save As dialog box appears prompting you for a file name and path for the notebook file.

If you are saving the worksheet to an existing notebook file, the notebook is updated to include the new worksheet or the changes to the existing worksheet. To save worksheets as non-notebook files, you must export them. Setting Worksheet Display Options Use the Options dialog box to set the default display settings for worksheets. Note: You can also change individual cells or blocks of cells using the Format Cells dialog box.

These custom formats remain even after editing options in the Options dialog box. To set worksheet display options:. Click the Worksheet tab. Options include: General. Select to turn Worksheet undo on or off, or to set SigmaPlot to display an error message if duplicate column titles appear when running transforms.

Turn Worksheet undo off if you are using a large data set and have a small amount of memory. Select to control how many decimal places you want to appear in the worksheet, or if you want to use E notation. For more information, see Changing Numbers Display below. Date and Time.

Select to set the display for the specified columns. For more information, see Changing Date and Time Display below. These buttons are available only if a Statistics worksheet is in focus. For more information, see Statistics Options below.

Set column widths, row heights, color and thickness of the worksheet grid lines, adjust data feedback colors, and select a font style and size. Freezing Panes You can freeze panes to keep rows and columns visible as you scroll through the worksheet. To freeze panes:. Select a cell below and to the right of where you want the split to appear.

From the menus select: Window Freeze Panes. Using Special Worksheet Shortcuts In addition to the menu commands and toolbar buttons referred to in the body of this manual, right-clicking the worksheet displays a right-click popup menu. Moving Around the Worksheet You can move around the worksheet using scroll bars or, move the highlighted worksheet cursor with the keyboard. Move to end of worksheet Home. Move to last row of last data block F2. Put cells into Edit mode.

Going to a Cell You can move the worksheet cursor to any cell in the worksheet by specifying the column and row number in the Go to Cell dialog box. The Go to Cell dialog box appears. Figure Moving to a Specific in the Worksheet. Enter the desired column and row number. To select the block of cells between the current highlight location and the new cell, click Extend Selection to Cell.

Click OK to move to the new cell. Using the Worksheet Shortcut Menu In addition to the menu commands and toolbar buttons, right-clicking the worksheet displays a shortcut menu.

Entering Data into a Worksheet This section describes entering data into worksheet columns, and formatting the columns for numeric, label, or date and time display. To enter data in a SigmaPlot worksheet:. Place the cursor in a cell. Type a number, label, or date and time value. Press Enter to move down one row, or use the arrow keys to move around the worksheet.

If you make a mistake entering data, click Undo on the Standard toolbar. For more information, see Undoing Mistakes in Chapter 1. Entering Dates and Times Enter dates and times using delimiters.

The delimiters used are determined by the Windows Regional Settings. For more information, see Regional Settings below. Date Delimiters.

The default date delimiter for most systems is a forward slash. An entry that displays only two fields of a date value is assumed to be day and month. If the second fields value is greater than 31, months and years are assumed. If you enter only two digits for the year, the century defined in your Regional Settings is implied. Time Delimiters. The default time delimiter is usually a colon :. Entries displaying two fields of a time value are assumed to be hours and minutes.

If PM is not specified, hours less than 12 are assumed to be morning hours. An entry with two colons assumes hours:minutes:seconds.

Insertion and Overwrite Modes Press the Insert key or use the Edit menu Insertion Mode command to switch between overwrite and insert data entry modes. If in Insertion Mode, Ins appears in the status bar. A check mark next to the Insertion Mode command on the Edit menu also indicates that the worksheet is in insertion mode. If in Insertion Mode, new data entered in a cell does not erase the previous contents.

Any existing data in the column is moved down one row. Pasting a block of cells pushes existing data down to make room for the pasted cells. If you cut or clear data, data below the deleted block moves up. If not in Insertion Mode, the worksheet is in overwrite mode. Data entered into a cell replaces any existing data.

If you paste a block of data, the block overwrites existing data. Importing Files from Other Applications You can import data from other applications into an existing worksheet for graphing, worksheet display, or running regressions. When you import data, it appears at the position of the worksheet cursor. For more information, see SPSS. SAV below. SigmaPlot 1. SigmaPlot Macintosh 4 Worksheet.

SigmaPlot Macintosh 5 Worksheet. SigmaStat 1. TableCurve 2D and 3D files. Microsoft Excel files. Lotus files. Plain Text files. For more information, see Importing Text Files below. Comma Delimited files. SigmaScanPro Worksheets. SigmaScan Image. Mocha Worksheets. Axon Text and Binary formats. For more information, see Importing Axon Files below. To import data:. Place the cursor to the worksheet cell where you want the imported data to start.

From the menus select: File Import. The Import File dialog box appears. Select the type of file you want to import from the Files of Type drop-down list. Change the drive and directory as desired, select the file you want to read, then click Import, or double-click the file name. Depending on the type of file, the data is either imported immediately, or another dialog box appears. Copying and Pasting Data from Other Applications Perhaps the easiest way to import data from another application is to simply copy and paste it from that applications spreadsheet into SigmaPlot.

This is perhaps the simplest method, especially if you cannot directly import the data into SigmaPlot. Once you have copied and pasted the data, you can promote the top row of data the variable names – to become the column titles. After defining the data source, you can then either import tables or import using SQL structured query language.

To define the ODBC data source:. To add a data source that is not on the list, click Add. Figure Adding a Data Source. Click the User DSN tab. Select a name from the User Data Sources list. Click Add. The Create New Data Source dialog box appears. Select a driver for which you want to set up a data source from the Name list, and click Finish. Enter a name to identify the new data source. Figure Identifying the Data Source. Click Select. The Select Database dialog box appears. Select the database, and click OK.

If the data source already appears in the User and System Sources drop down-list, select it. The Import Table dialog box appears. Figure Importing Fields from a Table. Select fields in the table by moving fields from Unselected fields to Selected fields by double-clicking a selection in the list. Click Import to import the fields into the worksheet. The Field names in the database become column headings in the worksheet. All records in the table are imported. Figure Data that has been Imported into a Worksheet.

Click Open to open an. Click Import to run the query and import the data. Field names in the database become column headings in the worksheet. Only the records defined by the SQL rows are imported. Importing Excel as ODBC When importing Excel spreadsheets using the ODBC Options dialog box, you must first assign a name to each data set or a range of data which is then imported as a table; otherwise, the Excel file will not import.

Select a range of data in the Excel spreadsheet. From the Excel menus select: Insert Name Define. In the Define Name dialog box, enter a name for the range of data in the Names in workbook box. Follow the steps above for as many data sets that you would like to create, and then save the Excel file. Now you can import this file as a database.

Select the range of data by specifying the start and end of the range; the default is the entire range. Click Import to place the data in the worksheet. From the menus select: File Import File.

Select an. The Import Spreadsheet dialog box appears. Figure Import Spreadsheet Dialog Box. Select either the entire spreadsheet or a specified range of cells. Specify cells using the standard Lotus notation for example, A1:C50 for a range from cell a1 to cell c When you have finished specifying the range to import, click Import. The selected data is imported. Note: The dialog box indicates whether or not the worksheet is in overwrite or insert mode, and where the imported data will begin.

To import spreadsheet data from non-compatible programs, save the spreadsheet as either an Excel or text file, then import that file. If you want to use an Excel workbook as an actual Excel workbook within SigmaPlot , you must open the workbook instead of importing it. Importing places the Excel data into a SigmaPlot worksheet, and does not open the workbook as an actual Excel workbook.

Use this dialog box to view the text file and to specify other delimiter types, or to build a model of the data file according to custom column widths. Figure Import Text Dialog Box. Note: A quicker method of importing text is copying the data in your source application, then opening SigmaPlot and pasting the data. To specify a different column separator, select Delimiter to activate the delimiter options; then select the appropriate type.

You can select commas, hyphens, or any other characters. For example, many databases use semicolons ; as delimiters. To specify a model of the data, use dashes – to specify column widths, and bracket characters [ and ] to define the column edges. Use a vertical bar character to indicate a single-character width column.

Click Analyze to re-display the appearance of the file using the new model. To save text import formats, enter a name into the Format scheme box, then click Add. Delete unwanted import formats using the Remove button.

To specify a different range, enter the rows and columns to read, then click Analyze. You can use this feature to eliminate file headers and other undesired text. When you are finished specifying the file parameters, click Import. The specified data from the file is imported. SigmaPlot imports both text and binary data files; if you select one of these options, the Import Axon dialog box appears prompting you to select a range of data to import.

The File selected is indicated in the dialog box title. Select the range of data by specifying the Row and Column ranges; the default is the entire range.

Click Import to place the data in the SigmaPlot worksheet. To select variables to import:. In the Unselected Variables list, select a variable you want to import. Note: SPSS data files use category data as the default data format. Exporting Worksheet Data Saving worksheets as non-notebook files is useful if you want edit your data in other spreadsheet applications.

Exporting worksheets does not export associated graphs. You can only export the entire worksheet. If you want to export a portion of the worksheet, delete the portion you do not want to export, then export the remainder of the worksheet.

How to Export a Worksheet 1. Select the worksheet you want to export by opening and viewing it, or selecting it in the notebook window. From the menus select: File Export. The Export File dialog box appears. Select a file format from the Files of type drop-down list, and then enter the file name, directory, and drive for the exported file. Click Export to create the file.

Exporting Worksheets as Text Files When you export a worksheet as a text file, tabs or commas are used to separate data columns and data is saved at full precision. If you want to save a text file with data as it appears in the worksheet rather than at full precision, copy the selected data to the Clipboard, paste it into a text editor, and save it as a text file.

Descriptive Statistics for Worksheets SigmaPlot automatically calculates a number of basic statistical values for all the data in your worksheet columns. For more information, see Printing Column Statistics. To view the statistics for the currently selected worksheet:. The running calculations performed for each column appear in a Column Statistics window for that worksheet. Figure Column Statistics Worksheet. Available Statistics To determine the statistics shown in the Statistics windows, use the Statistics Options dialog box.

Most calculations ignore empty cells, missing values, and text. The following statistics appear in the Column Statistics window. The arithmetic mean, or average, of all the cells in the column, excluding the missing values.

This is defined by. Std Dev. The sample standard deviation is defined as the square root of the mean of the square of the differences from their mean of the data samples xi in the column. Missing values are ignored. Std Err. The standard error is the standard deviation of the mean.

It is the sample standard deviation divided by the square root of the number of samples. For sample standard deviations. The number of occupied cells in the column, whether they are occupied by data, text, or missing values. The arithmetic sum of the data values in the column.

The value of the numerically smallest data value in the column, ignoring missing values. The value of the numerically largest data value in the column.

Min Pos. The smallest positive value. The number of cells in the column occupied by missing values, denoted with a double dash symbol Either text or an empty cell. Statistics Options To display only a portion of the available statistics, use the Worksheet Options dialog box, then select the column statistics to show or hide.

To specify which statistics are shown or hidden:. Select the statistic s you want shown or hidden. Select the appropriate options to change the column widths and data display.

The E expresses the power of For example, 1. Engineering Notation, which you can select as an option on the Worksheet tab of the Options dialog box, uses integral powers of 3 with 10 as the base. Figure Figure Numbers are displayed in Column 1, dates are displayed in Column 2, and text is shown in Column 3. You can enter numbers, labels, and dates and times directly into the worksheet.

You can also convert numbers to dates and times and vice versa. You can change column widths, number decimal places, or date and time format, and you can also change the color and thickness of the worksheet gridlines, and adjust data feedback colors.

Note: You can format columns to override the defaults set using the Options dialog box. Sizing Columns and Rows If the contents of your column exceed the column width, cell contents display as pound symbols.

Label entries are truncated. To change a column width:. Drag the boundary on the right side of the column heading until the column is the size you want. To change a row height:. In the Settings For list, click Appearance. Set column width and row height in the Column Width and Row Height drop-down lists. Click OK to apply the changes and close the dialog box. SigmaPlot s worksheet can display up to fourteen digits of precision regardless of how many decimal places you specify. Changing the Appearance of the Worksheet Grid You can change the color and thickness of worksheet grid lines.

To change the grid appearance:. Set color and thickness in the Color and Thickness drop-down lists. Setting Data Feedback Colors Data Feedback highlights the cells and columns on the worksheet that correspond to the X and Y values of the selected curve or data point.

You can change these colors on the Options dialog box. To change the data feedback colors:. Set data feedback colors and thickness in the X and Y drop-down lists. Setting Decimal Places To set the number of decimal places used for worksheet values: 1. In the Settings For list, click Numeric. Select the number of decimal places from the Decimal Places drop-down list. Click OK to accept the changes and close the dialog box. If the number of decimal places exceeds the column width they appear as symbols.

E Notation When Needed Displays worksheet data as scientific notation only when the length of the value exceeds the width of the cell. The default column width is twelve. The number of decimal places is set in the Decimal Places edit box. Set the number of decimal places in the Decimal Places edit box. The number of decimal places allowed is limited by the column widththe maximum number of decimal places cannot exceed the column width or it appears as a series of symbols.

The default setting for decimal places is two. Select a Numeric format setting from the Display As drop-down list. To use engineering scientific notation for worksheet values, select Engineering Notation. For more information, see Engineering and E Notation above.

Click OK to accept the settings and close the dialog box. For example, if you enter a day and month, you can display the month and year. To view and modify the current settings:. Select the Date and Time from the Settings for list. Complete month dd-MMM-yy. October 08, dddd, dd MMMM, yyyy. Era AD or BC 5. To change the display Time format, type one of the following examples into the Time box, or select a format from the drop-down list: hh or h.

Military hours mm or m. Minutes ss or s. Seconds uu or u. Milliseconds H: h: m: s: or u. No leading zeroes for single digits HH: hh: mm: ss: uu. Leading zero for single digits tt. Double letter AM or PM t. Single letter AM or PM. Setting a Start Date is only necessary if you are importing numbers to be converted to dates, or converting dates to numbers for export. The starting date must match the date used by the other application. Note that SigmaPlot recognizes day zero as starting at 0.

Some spreadsheet software products begin day zero at 1. SigmaPlot now employs an all new user interface allowing users to easily setup a global curve fit. This gives users the ability to easily share one or more equation parameters across multiple data sets. Non-linear curve fitting is known to produce incorrect results in some instances. Dynamic Curve Fitting is designed to determine if this has happened and if so what the likely best fit is.

Viewers can explore data used to create graphs and zoom, pan or print images at full resolution directly from a Web Browser. Automatically generate active Web objects from your graphs or embed the objects within other Web pages. Each worksheet can hold a list of user defined transforms that will automatically be re-run whenever the transform input data has changed. SigmaPlot lets the user select a graph first and then gives you a pre-formatted worksheet to structure their data.

The data entered into the worksheet is immediately displayed on the graph. This feature can demonstrate to you the strong relationship between the data format and the graph type.

Take advantage of ribbon collections of common properties, tabbed selection of graphs, worksheets and reports, right mouse button support and graph preferences.

The interactive Graph Wizard leads you through every step of graph creation. You get compelling, publication-quality charts and graphs in no time. SigmaPlot offers more options for charting, modeling and graphing your technical data than any other graphics software package. Compare and contrast trends in your data by creating multiple axes per graph, multiple graphs per page and multiple pages per worksheet.

SigmaPlot offers the flexibility to customize every detail of your graph. You can add axis breaks, standard or asymmetric error bars and symbols; change colors, fonts, line thickness and more. Double-click on any graph element to launch the Graph Properties dialog box.

Modify your graph, chart or diagram further by pasting an equation, symbol, map, picture, illustration or other image into your presentation. Save all of the attributes of your favorite graph style in the Graph Style Gallery. Add greater speed and efficiency to your analysis by quickly recalling an existing graph type you need and applying its style to your current dataset.

In the Notebook Manager, you can copy and paste a graph from one worksheet to another and all the attributes of that graph are applied to the new data saving much time. Create stunning slides, display your graphs in reports or further customize your graphs in drawing packages. Presenting and publishing your results has never been easier — or looked this good. Then, just double click your graph to edit directly inside your document. Quickly send your high-resolution graphs online to share with others.

Viewers can explore data used to create vector graphs and zoom, pan or print images at any resolution directly from a Web Browser. SigmaPlot provides all the fundamental tools you need to analyze your data from basic statistics to advanced mathematical calculations.

You can fit a curve or plot a function and get a report of the results in seconds. Use built-in transforms to massage your data and create a unique chart, diagram or figure. Access SigmaPlot right from your active Microsoft Excel worksheet.

Use Excel in-cell formulas, pivot tables, macros and date or time formats without worry. Keep your data and graphs in one convenient file. Generate simulated data or modify worksheet columns of data with transforms. Create simple one-line transforms with the Quick Transforms feature that walks you through transform implementation. Or create extremely complex transforms with hundreds of lines of code. Fitting your data is easy with the SigmaPlot Regression Wizard. The Regression Wizard automatically determines your initial parameters, writes a statistical report, saves your equation to your SigmaPlot Notebook, and adds your results to existing graphs or creates a new one!

The Regression Wizard accurately fits nearly any equation — piecewise continuous, multifunctional, weighted, Boolean functions and more — up to 10 variables and 25 parameters. You can even add your own curve fit equations and add them to the Regression Wizard. The Dynamic Curve Fitter performs or more curve fits using your equation and data starting from optimally different initial starting values. The results are ranked by goodness of fit so that you can check the top ranked results against the result you obtained from the Regression Wizard.

For many simple equations, which are fit to data sets with a sufficiently large number of data points, the Dynamic Curve Fitter finds the same result as the Regression Wizard.

But the problem is that the user simply does not know whether the solution found by the Regression Wizard is the best possible or not. So there is always a concern that the correct solution has not been found. Dynamic fitting minimizes this concern. Its use is encouraged prior to publishing results particularly if a complicated equation is used.

Plotting user-defined and parameterized equations is only a mouseclick away with the Plot Equation feature. Just type the function or select one from the built-in library and specify the parameters and the range. Create your own built-in functions and save them for future use. Plot functions on new or existing graphs or plot multiple functions simultaneously using different parameter values. Save plotted X and Y results to the worksheet.

Not a programmer? No problem. With SigmaPlot, you can record macros by point-and-click with the macro recorder. Use macros to acquire your data, execute powerful analytical methods, and create industry-specific or field-specific graphs.

Use one of the thirty built-in macros as provided or use these macros as a base to quickly create your own macros. Share the power of SigmaPlot with less-experienced users by using macros to tailor the SigmaPlot interface for your particular application.

Create custom dialog boxes, menu choices and forms to help guide novice users through a session. Analyze and graph your data using SigmaPlot within those applications. SigmaPlot is now a complete graphing AND an advisory statistics suite. All of the advanced statistical analysis features found in the package known as SigmaStat have now been incorporated into SigmaPlot along with several new statistical features.

SigmaPlot guides users through every step of the analysis and performs powerful statistical analysis without the user being a statistical expert. In addition to the EC50 value already computed, the user can also compute other user-entered EC values such as EC40 and EC60 and compute them instantly.

Two five-parameter logistic functions have also been added and the Dynamic Curve Fitting feature included to help solve difficult curve fitting problems. In earlier versions of SigmaPlot, almost all objects in a 2D graph were selectable with just a mouse click.

However, almost all objects in a 3D graph were not. SigmaPlot now adds mouse selectability of all 3D graph objects with the ability to customize all 3D objects. Just double click your graph to edit directly inside your document. Skip to content. SigmaPlot for Graphing and Data Visualization.

Learn More. SigmaPlot Instrumentation Framework. More than 2-D and 3-D technical graph types. Use Global Curve Fitting to simultaneously analyze multiple data. Obtain Data from Nearly Any Source. SigmaPlot Features. Choose from a wide range of graph types to best present your results.

Statistical Analysis is no longer a daunting task. Simply select the Web graph to share its data with colleagues and students Share the data behind your graphs with colleagues and students Enable colleagues to print your full report from your intranet or Web site directly from their browsers — without compromising the quality of the graphs Create an optional password while exporting your graph to limit data access to authorized users Produce Web documents without knowing HTML or embed SigmaPlot Web object graphs within HTML files to create interactive electronic reports Each worksheet can hold a list of user defined transforms that will automatically be re-run whenever the transform input data has changed.

Graphing software that makes data visualization easy. Customize every detail of your charts and graphs. Publish your charts and graphs anywhere. Share high-quality graphs and data on the Web.


Sigmaplot 12.5 user guide free. Sigma Plot Statistics User Guide


Thank you sigmaplot 12.5 user guide free visiting nature. You adobe premiere pro cc online free using a browser version with limited support for CSS.

To obtain the best experience, we recommend you use a more up to date читать далее or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we sitmaplot displaying the site without styles and JavaScript. Circular dichroism CD guidee an sigmaplot 12.5 user guide free tool for rapid determination of the secondary structure and folding properties of proteins that have been obtained using recombinant techniques or purified from tissues.

The most widely used applications of protein CD are to determine whether an expressed, purified protein is folded, or if a mutation affects its conformation or stability. In addition, it sigmaplot 12.5 user guide free be used to study protein interactions. This protocol details the basic steps of obtaining and interpreting CD data, and methods for analyzing spectra to estimate the secondary structural composition of proteins. However, it does not give the residue-specific information that can be obtained by x-ray crystallography or NMR.

Your institute does not have access to this article. Scientific Reports Open Access 13 July Scientific Reports Open Access 14 February Velluz, L. Google Scholar. Beychok, S. Circular dichroism of biological macromolecules. Science— Sreerama, N. Computation and analysis of protein circular dichroism spectra. Methods Enzymol. Holzwarth, G. The ultraviolet circular dichroism of polypeptides.

Greenfield, N. Computed circular dichroism spectra for the evaluation of protein conformation. Iser 8— Venyaminov, S. Circular dichroic analysis of denatured proteins: inclusion of guire proteins in the reference set. Bovey, F. Circular dichroism sigmaplott of poly-L-proline.

Biopolymers 5— Tiffany, M. Effect of temperature on the circular dichroism spectra of polypeptides in the extended state. Biopolymers 11— Woody, R. Circular dichroism and conformation of unordered polypeptides. Rucker, A. Polyproline II helical structure in protein unfolded states: lysine peptides revisited. Protein Sci. Nafie, Sigmaplot 12.5 user guide free. Infrared and Raman vibrational optical activity: theoretical and experimental aspects. Jackson, M. The use and смотрите подробнее of FTIR spectroscopy in the determination of protein structure.

Cooper, E. Fourier transform infrared spectroscopy investigations of protein structure. Pelton, J. Spectroscopic methods for analysis of protein secondary structure. Lowry, O. Protein measurement with the Folin phenol reagent. Bradford, M. Guidw rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein—dye binding.

Hennessey, J. Information content in the circular dichroism of proteins. Biochemistry 20— Lang, C. Simple microdetermination of Kjeldahl nitrogen in biological material. Jaenicke, L. A rapid micromethod for the sigmaplit of nitrogen and phosphate in biological material. Brahms, S. Determination of protein secondary structure in solution by vacuum ultraviolet circular dichroism. Reed, J. A set of constructed type spectra giide the practical estimation of peptide secondary sigmaplot 12.5 user guide free from circular dichroism.

Saxena, V. A new basis for interpreting the circular dichroic spectra of proteins. Вот ссылка Acad. USA 68— Uxer, Y. Biochemistry 13— Chang, C. Yang, J. Calculation of protein conformation from circular dichroism. Methods to estimate the conformation of proteins and polypeptides from circular dichroism data. Provencher, S. Estimation of globular protein secondary structure from circular dichroism. Biochemistry 2033—37 Manavalan, P.

Variable selection method improves the prediction of protein secondary structure from circular dichroism spectra. Toumadje, A. Extending CD spectra of proteins to nm improves the analysis for secondary structures.

Effects of relative band intensity on prediction of protein secondary structure from CD. Johnson, W. Analyzing protein circular frde spectra for accurate secondary structures. Proteins 35— A self-consistent method for the analysis of protein secondary structure from circular dichroism. Protein secondary structure from circular dichroism spectroscopy. Combining variable selection principle sigmplot cluster analysis with neural network, ridge regression and self-consistent methods. Estimation of protein guie structure from circular dichroism spectra: inclusion of denatured proteins with native proteins in the analysis.

Quantitative sigmaplot 12.5 user guide free of protein far UV kser dichroism spectra by neural networks. Sigmapllt Eng. Article Google Scholar. Andrade, M.

Evaluation of secondary structure of proteins from UV circular dichroism spectra using an unsupervised sigmaplpt neural network. Unneberg, P. Proteins 42— Perczel, A. Analysis of the circular dichroism spectrum of proteins using the convex sigmaplot 12.5 user guide free algorithm: a practical guide. A new approach to the calculation of secondary structures of globular proteins by optical rotatory dispersion and circular dichroism.

Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion. Biochemistry 11— Poly pro II helices in globular proteins: identification and circular dichroic analysis.


Sigma Plot 11 Users Guide | PDF | Databases | Cartesian Coordinate System

Create SigmaPlot graphs using Microsoft Excel You can use SigmaPlot ing axis labels, titles, legend text or free-form text. SigmaPlot guides users through every step of the analysis and performs powerful statistical analysis without the user being a statistical expert. In addition to.


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